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Dark Field Microscope OPTIKA UHD-PLUS Set

Product number: OPTIKA-SET-UHD-PLUS
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Dark Field Microscope UHD-PLUS-Set

The UHD-PLUS-Set is our dark field system with LED technology including camera and monitor in UHD (Ultra High Definition) resolution - that means 3840x2160 pixels. It offers vital blood observation through the eyepieces as well as live view and the ability to record images and videos through the integrated camera. It is the recommended choice for users who value the highest image quality.

The camera supplied has two outputs: an HDMI output for direct connection to the supplied monitor (recommended type of use) and a USB output for optional use with a Windows PC. You can even use both outputs at the same time. HDMI mode gives you the highest frame rate and quality. In this mode, pictures and videos are recorded on the supplied and easily removable SD memory card on the camera and can then be transferred to the computer or played back directly from the camera to the monitor - the camera has an image gallery function with the ability to display pictures/videos!

The camera's c-mount adapter included in the set has built-in optics and a focusing ring. After adjusting the focus ring, the image through the eyepieces and on the screen gets displayed sharply at the same time, so no annoying re-adjustment is necessary. This adjustment would not be possible with cheaper adapters with a fixed focal length and without a focusing ring.

Set content:

  • Dark field microscope OPTIKA B-510DK
  • Microscope camera adapter OPTIKA M620.1
  • Microscope camera HDC DF4L
  • Monitor Samsung 31.5" UHD

Below is the more detailed description of the microscope and the camera.

Dark field microscope OPTIKA B-510DK

The darkfield microscope OPTIKA B-510DK belongs to the OPTIKA 500 series; This product line includes models with excellent performance, designed for laboratory use. It is ultra-modern (LED technology), robust (die-cast frame), ergonomic and is mainly suitable for the vital blood examination . It can be combined via adapter with microscope and/or SLR cameras for live viewing and microscopic recordings.


Trinocular observation head, 360°rotatable, 30° inclined


Plan extra wide field, PL 10X/22


  • IOS Planachromatic 4X, A.N. 0.10, W.D. 17,3 mm
  • IOS Planachromatic 10X, A.N. 0.25, W.D. 10 mm
  • IOS Planachromatic 40X, A.N. 0.65, W.D. 0,54 mm
  • IOS Planachromatic 100x with iris, A.N. 0.36-1.25, W.D. 0,18 mm (oil immersion)


Quintuple revolving nosepiece, rotation on ball bearings


Double layer with mechanical sliding stage, size: 233x147 mm, X-Y movement range 78x54 mm. Belt-drivein X direction. Vernier scale on two axes, accuracy 0.1 mm


Coaxial coarse and fine focusing mechanism (graduated 0.002 mm)


  • Darkfield condenser N.A. 1.36 with integrated X-LED
  • Bright field condenser


LED-Technology, color temperature: 6.300K, LED average lifetime: ca. 50.000 hours of operation

Technical Specifications

Please click on the "MEDIA" tab to find the manufacturer's technical specifications.

Sample captures

The following video was taken through the trinocular tube with the 40X lens (total magnification: 400X)

HDMI/USB microscope camera HDC DF4L

The DF4L model is the right choice for users who, on the one hand, expect very high quality standards for image quality and, on the other hand, want to remain flexible with regard to system compatibility. Good image quality is guaranteed by the built-in SONY IMX334 (C) CMOS color image sensor, which enables pictures in a resolution of 3840 x 2160. In terms of compatibility, the camera can be operated with or without a PC and can be used in one of the following operating modes: HDMI (without PC) and USB (with Windows PC).

The most important functions of the camera can be found below - functions marked with (*) are only available using the supplied Windows software - and not in pure HDMI mode.

Multi-signal output : The camera can either be connected directly to an HDMI monitor (via the HDMI connection, without a PC in between) or alternatively to a PC (via the USB connection). In both modes, the camera offers high refresh rates to track and record microscopic movements. However, the camera can also be used in mixed mode by working on the PC and additionally transmitting the image via the HDMI output to a second HDMI monitor.

Flexible exposure times : In addition to the auto exposure, you can also set the exposure time (exposure) and the gain (gain) manually depending on the application. The exposure time can be set almost continuously up to 1 sec (gradation: 1 ms).

ROI - Flexible White Balance : You can choose between automatic and manual white balance. In addition, you can activate an automatic white balance with reference to a selectable image region (ROI - region of interest). In the ROI white balance mode, you frame a rectangular area on the image (rectangle can be moved and size can be adjusted with the mouse). The software then only takes over this area as a reference for white.

Auto-Stitch * : The software can combine several partial overlapping images into one overall image at the push of a button. We generated the sample image below from five images (overlapping area between images of 10-15%) fully automatically.

Auto-stitch function of the HDC DF4 microscope camera

Extended Depth of Field * (EDF) : Sometimes it is not possible to focus on the entire area because the depth of field is limited by the optics. After the user has taken several images with different focus settings, the software uses stacking to create a superimposed image that only takes over the sharp areas of each individual image. We had the sample image below/right generated fully automatically from four partial shots. The partial shots only partially contained sharp areas such as can be seen in the image on the left.

EDOF function of the HDC DF4 microscope camera

In the sample below using the EDOF function we could capture sharp within one image the most part of the insect.

sample capture of a fly using  EDOF with the HDC DF4 microscope camera

HDR - High Dynamic Range*: This function merges a sequence of differently exposed images into one image with a higher dynamic range. In the example below, we have created a new image with ideal exposure for each area of ​​the image from two images, each with under and overexposure.

HDR function of the HDC DF4 microscope camera

Zoom - Mirror - Compare - Freeze : You can use the software to zoom in and out of the image and mirror the image horizontally / vertically. Furthermore, you can divide the screen vertically in two halves: the left half shows the live image and the right half an existing image from your image gallery to compare preparations. You can also freeze the live image and then release it again for the viewer.

Zoom and comparison function Control Function

Measurements: The camera software can carry out measurements after user calibration

Interval Recording *: Interval recordings can be programmed, whereby the time interval between the single image captures and their total number can be selected. With this e.g. biological decay processes can be documented over time.

Built-in Mouse Control (HDMI mode) is a special feature of this model: the included mouse is connected to the USB port of the camera. With the mouse you can then operate the on-screen display menu and use it to take pictures and videos and make all camera settings - completely without a PC and software installations!

Mouse Control Function

Video recording on SD card (HDMI mode): Videos and pictures can be recorded in 4K quality directly on the removable SD card.

Built-In Player : (HDMI mode): Images and videos already recorded are saved on the SD card of the camera. You can scroll through them and have the camera play them on the monitor - without a PC!

Sample captures

In the following video you can see a recording of the DF4L; it shows the movement of mainly red blood cells in the blood (dark field, magnification: 400X, OPTIKA B-510DK microscope)

In the following picture you can see a picture of the mouth tool of a honey bee (bright field, magnification: 100X, OPTIKA B-510DK microscope).

Musteraufnahme Honigbiene

In the following picture you see a picture of a plant section (bright field, magnification: 100X, OPTIKA B-510DK microscope).

Musteraufnahme Pflanze

Windows User Interface

The multilingual user interface of the Windows software supplied offers on the one hand, the control options for the camera parameters (exposure, gain, recording) and on the other hand, options for image correction (white balance, color tones, etc.) as well as access to the extended functions (measurement, extended depth of field, auto-stitch etc.) .)

User Interface ImageView Software

Technical data

  • Sensor: Sony IMX334 (C), sensor size 1/1,8"
  • Image capture resolution: 3840 × 2160
  • Refresh rate (HDMI, USB): 30, 20 fps
  • Exposure time (sec): 0.004 - 1
  • White balance: auto, manual, auto with selectable ROI (Region Of Interest)
  • Language support of ImageView Windows software: German, English, French, Italian, Spanish
  • Special functions of ImageView: auto-stitch, EDF (extended depth of field), HDR, segmentation, time lapse, measurements
  • Outputs: HDMI, USB, SD card
  • Compatibility: Windows 10 (32/64 bit)
  • Connection: C-Mount
  • Weight (g): 470
  • Dimensions (cm): 8,5 x 7,8 x 6,5
  • Technical data sheet (PDF): see tab "Media"

Scope of delivery

  • Camera
  • Software ImageView for Windows
  • HDMI cable
  • USB cable
  • Power supply 12V / 1A
  • USB mouse
  • SD card 16GB
  • Operating instructions (German)
  • Software user guide (English)

Manufacturer's Guarantee

  • 18 months from the date of purchase (EU)